Journal: Poultry science

Article Title: Effect of transient receptor potential vanilloid 6 gene silencing on the expression of calcium transport genes in chicken osteoblasts.

doi: 10.3382/ps/peu071

Figure Lengend Snippet: Figure 3. Expression levels of calcium transport genes in chicken osteoblasts 48 h after transfection. Levels were quantified by real-time PCR. (A) Expression levels of TRPV6. The data were expressed as the means ± SEM. ∗P < 0.05 and ∗∗P < 0.01 compared with the untreated group. (B) Expression levels of calbindin-D28K, PMCA1b and NCX1 after the transfection with pSIREN-TRPV6-3. The data were expressed as the means ± SEM. ∗∗P < 0.01 compared with the untreated group.

Article Snippet: The blots were incubated with the primary antibodies anti-calbindin-D28K (BM0203, Boshide, China), anti-TRPV6 (ACC-036, Alamone Labs, Israel) and anti-β-actin (Santa Cruz), which were diluted 1:500, 1:500, and 1:2000, respectively.

Techniques: Expressing, Transfection, Real-time Polymerase Chain Reaction

Journal: Poultry science

Article Title: Effect of transient receptor potential vanilloid 6 gene silencing on the expression of calcium transport genes in chicken osteoblasts.

doi: 10.3382/ps/peu071

Figure Lengend Snippet: Figure 4. Expression of TRPV6 and calbindin-D28K proteins in chicken osteoblasts 48 h after transfection. The protein expression levels of TRPV6 and calbindin-D28K were reduced by 40.2% (P < 0.01) and 29.8% (P < 0.01), respectively, 48 h after transfection with pSIREN-TRPV6-3 compared with the level obtained in the untreated group. There was no significant difference between the untreated and pSIREN-control groups. Representative blots for each group from trip- licate experiments were shown. The protein band density was quan- tified by densitometry using the Quantity One software. The protein level was expressed as a ratio relative to the expression of β-actin. The data were expressed as the means ± SEM. ∗∗P < 0.01 compared with the untreated group.

Article Snippet: The blots were incubated with the primary antibodies anti-calbindin-D28K (BM0203, Boshide, China), anti-TRPV6 (ACC-036, Alamone Labs, Israel) and anti-β-actin (Santa Cruz), which were diluted 1:500, 1:500, and 1:2000, respectively.

Techniques: Expressing, Transfection, Control, Software

Journal: Cerebellum (London, England)

Article Title: The Primary Ciliary Deficits in Cerebellar Bergmann Glia of the Mouse Model of Fragile X Syndrome

doi: 10.1007/s12311-022-01382-8

Figure Lengend Snippet: (A) Immunostaining of Arl13b (red), NeuN (cyan, top), calbindin (green, middle), and BLBP (yellow, bottom) with DAPI nuclear staining (grey) in the posterior cerebellum of adult WT (left) and Fmr1 KO (right) mice. Blue arrows, Arl13b+ cells; Magenta arrowheads, Arl13b− cells. Scale bar, 10 μm. (B) Quantification of the percentage of Arl13b+ cells among NeuN+ cells (top), calbindin+ cells (middle), or BLBP+ cells (bottom) in the cerebellum from adult WT and Fmr1 KO mice. (C) Immunostaining of Arl13b (red) and BLBP (yellow) with DAPI nuclear staining (grey) in the PCL of P7, P10, P14, P28, and adult WT (left) and Fmr1 KO (right) mice. Blue arrows, Arl13b+ cells; Magenta arrowheads, Arl13b− cells. Scale bar, 10 μm. (D) Quantification of the percentage of Arl13b+ cells among BLBP+ cells in the cerebellum from WT and Fmr1 KO mice. n=4–8 for each group (A), n=8–24 for each group (C), mean ± SEM. Student’s unpaired t-test. *P<0.05; **P<0.01; ***P<0.001.

Article Snippet: The following primary antibodies were used in this study: mouse anti-BLBP (1:200; Abcam; ab131137), mouse anti-Sox-2 (1:100; Santa Cruz; sc-365964), mouse anti-S-100β chain (1:100; Santa Cruz; sc-393919), mouse anti-NeuN (1:500; Millipore-Sigma; MAB-377), chicken anti-calbindin (1:400; Encor; CPCA-Calbindin), rabbit anti-Arl13b (1:700; Proteintech; 17711–1-AP), rabbit anti-BLBP (1:300; Abcam; ab32423), rat anti-BrdU (1:200; Novus; NB500–169), and rat anti-BrdU (1:200; Abcam; ab6326).

Techniques: Immunostaining, Staining

Journal: Poultry science

Article Title: Effect of transient receptor potential vanilloid 6 gene silencing on the expression of calcium transport genes in chicken osteoblasts.

doi: 10.3382/ps/peu071

Figure Lengend Snippet: Figure 3. Expression levels of calcium transport genes in chicken osteoblasts 48 h after transfection. Levels were quantified by real-time PCR. (A) Expression levels of TRPV6. The data were expressed as the means ± SEM. ∗P < 0.05 and ∗∗P < 0.01 compared with the untreated group. (B) Expression levels of calbindin-D28K, PMCA1b and NCX1 after the transfection with pSIREN-TRPV6-3. The data were expressed as the means ± SEM. ∗∗P < 0.01 compared with the untreated group.

Article Snippet: The blots were incubated with the primary antibodies anti-calbindin-D28K (BM0203, Boshide, China), anti-TRPV6 (ACC-036, Alamone Labs, Israel) and anti-β-actin (Santa Cruz), which were diluted 1:500, 1:500, and 1:2000, respectively.

Techniques: Expressing, Transfection, Real-time Polymerase Chain Reaction

Journal: Poultry science

Article Title: Effect of transient receptor potential vanilloid 6 gene silencing on the expression of calcium transport genes in chicken osteoblasts.

doi: 10.3382/ps/peu071

Figure Lengend Snippet: Figure 4. Expression of TRPV6 and calbindin-D28K proteins in chicken osteoblasts 48 h after transfection. The protein expression levels of TRPV6 and calbindin-D28K were reduced by 40.2% (P < 0.01) and 29.8% (P < 0.01), respectively, 48 h after transfection with pSIREN-TRPV6-3 compared with the level obtained in the untreated group. There was no significant difference between the untreated and pSIREN-control groups. Representative blots for each group from trip- licate experiments were shown. The protein band density was quan- tified by densitometry using the Quantity One software. The protein level was expressed as a ratio relative to the expression of β-actin. The data were expressed as the means ± SEM. ∗∗P < 0.01 compared with the untreated group.

Article Snippet: The blots were incubated with the primary antibodies anti-calbindin-D28K (BM0203, Boshide, China), anti-TRPV6 (ACC-036, Alamone Labs, Israel) and anti-β-actin (Santa Cruz), which were diluted 1:500, 1:500, and 1:2000, respectively.

Techniques: Expressing, Transfection, Control, Software

Journal: PLOS One

Article Title: Impaired dynein function preserves spinal interneuron survival and positioning in an ALS-like mouse model

doi: 10.1371/journal.pone.0346246

Figure Lengend Snippet: Number of ChAT + (A-C), GAD-67 + (D), Calbindin + (E), Parvalbumin + (F), Parvalbumin + −Calbindin + (G), Parvalbumin + −GAD-67 + (H), and ChAT + −GAD-67 + (I) cells per biological replicate. The number of cells analysed is the sum of immunostained positive cells from eight spinal cord cross-sections (two sections per lumbar level L3–L6) per biological replicate. Data are shown as mean ± standard deviation and were analysed by an ordinary one-way ANOVA. N = 3 biological replicates per genotype.

Article Snippet: Following three 10-min PBS washes at 100 rpm, secondary probing was performed for 3 hours in the dark in PBS containing 0.05% Tween-20 and the relevant secondary antibodies for ChAT (1:250 dilution, donkey anti-goat IgG H + L Alexa Fluor ® 405, Abcam, ab175664), Calbindin (1:500 dilution, Rhodamine TRITC-AffiniPure donkey anti-guinea pig Ig G H + L, Jackson Immuno Research, 706-025-148-JIR), Parvalbumin (1:500 dilution, Alexa Fluor 647-AffiniPure donkey anti-rabbit IgG H + L, Jackson Immuno Research, 711-605-152), and GAD-67 (1:500 dilution, donkey anti-chicken IgY H + L Highly Cross Adsorbed Alexa Fluor ® 488, Life Technologies, A78948).

Techniques: Standard Deviation

Journal: Frontiers in Neural Circuits

Article Title: Spatial distribution of D1R- and D2R-expressing medium-sized spiny neurons differs along the rostro-caudal axis of the mouse dorsal striatum

doi: 10.3389/fncir.2013.00124

Figure Lengend Snippet: Striatal interneurons distribution in the D2R/A2aR-expressing MSNs-poor zone of the caudal striatum . Striatal sections at the caudal level from Drd2-EGFP mice ( n = 5 for each staining) labeled with antibodies for choline acetyltransferase (ChAT, A ), parvalbumin (ParV, B ), neuropeptide Y (NPY, C ) and calretinin (CalR, D ). Scale bars, 200 μm. Insets, higher magnification (GFP, green, A 1 ; ChAT, magenta, A 2 ; merge, A 3 ), (GFP, green, B 1 ; ParV, magenta, B 2 ; merge, B 3 ), (GFP, green, C 1 ; NPY, magenta, C 2 ; merge C 3 ) and (GFP, green, D 1 ; CalR, magenta, D 2 ; merge D 3 ). Scale bars, 100 μm. DStr, dorsal striatum; GPe, external globus pallidus, GFP, green fluorescent protein.

Article Snippet: Finally, they were incubated overnight or 72 h at 4°C with the primary antibodies: chicken and rabbit anti-GFP (1:500 and 1:1000 respectively, Invitrogen), rabbit anti-vesicular glutamate transporter 1 (VGluT1) or anti-VGluT2 (1:1000 gift from S. El Mestikawy), mouse anti-tyrosine hydroxylase (TH) (1:1000, Millipore), rat anti-dopamine transporter (DAT) (1:1000, Millipore), mouse anti-NeuN (1:500, Millipore), mouse anti-D1R (1:500 gift from R. R. Luedtke), rabbit anti-Gα olf (1:500) (Hervé et al., ), rabbit anti-β-galactosidase (1:1000, Cappel, MP Biomedicals), guinea-pig anti-MOR (1:500 gift from T. Kaneko) mouse anti-DARPP-32 (1:1000 gift from P. Greengard), rabbit anti-calretinin (CalR), anti-calbindin-D28k and anti-parvalbumin (ParV) (1:1000, Swant), rabbit anti-neuropeptide Y (NPY) (1:400, Abcam), goat anti-ChAT (1:400, Millipore), rabbit anti-substance P (1:500, Millipore), rabbit anti-preproenkephalin (ppENK) (1:500, Neuromics) and rabbit anti-RFP (1:1000, MBL).

Techniques: Expressing, Staining, Labeling